Cytotoxic and genotoxic effects of gutkha

Review on aetiology and pathogenesis.

Arecanut as an emerging etiology of oral cancers in India

Any individual having multiple habits were also not included. It seems that there is an association between oral inflammatory conditions and the age of individuals who use smokeless tobacco products [ 95 ].

Formation of areca nut, and pan masala. When the alkaloids are compared on a weight basis with the extract, no single agent has detectable effects on the cells at concentrations of the extract that cause decrease colony survival and DNA single-strand breaks.

Value of Micronuclei Count as a Predictor of Cytotoxic Damage in Smoked and Smokeless Tobacco Users

Oral cancer is the sixth most predominant type of cancer worldwide, affecting both genders equally, although it is particularly common in men in developing countries [ 6 ]. PAP staining was done and the number of cells with micronuclei was counted under 40 X, by two independent examiners.

Few studies reported an elevation of MN count in exposed individuals as compared with control groups, but the observed effects were relatively small, ranging between 1.

Studies conducted on betel quid and tobacco use among South Asian emigrants to Western countries have pointed out the lack of awareness of oral cancer risk regardless of gender, age, national group, and social class.

Stich HF, Stich W. Multiple genes are involved in the breakdown of carcinogens, and the most frequently observed evidence has suggested that cytochrome polymorphisms CYPs affect the risk of oral cancer.

Studies have shown that tobacco users who include slaked lime in betel quid or with areca nut experience carcinogenic and genotoxic effects in human oral epithelium cells.

Certainly, gutkha producers are on the defensive [ 20 ]. The lack of betel quid leaf in paan and the higher levels of areca nuts may facilitate the development of OSMF in paan and gutkha users [ 21 ].

Taiwan Traditionally, tobacco has been consumed as part of the culture in some countries of the world. The benefits of this technique are that it is a fast and simple method and convenient for numerous substances. Therefore, additive or synergistic effects could be considered among the alkaloid.

It is produced in a chewable form and is also the main component of various products used daily by the younger population.

Essentially, areca nut is the fruit derived from Areca catechu. Among users, the youngest was of 12 years and the oldest one was of 65 years of age. Micronuclei MN count is a promising indicator for the cytotoxic effects of tobacco usage. The period of the individual in the buccal region of 30 cases and 30 controls study was almost 6 months.


For chromosome preparations cells were harvested by replacing the medium with 2 ml of collagenase solution 0. Thereafter, cells were incubated for an additional 48 h.The genotoxic effects of gutkha and pan masala are most likely due to the tobacco and areca nut specific nitrosamines.

For the evaluation of the genotoxic effects of various compounds using the MN assay, Cytotoxic and genotoxic effects of areca nut-related compounds. Genotoxic effects occur from paan and gutkha mostly due to the presence of nitrosamine, as shown in Table 3.

The nitrosamine in the chewers’ saliva undergoes nitration during betel quid chewing when it reacts with nitrite in the presence of a catalyst [ 42, 64 ]. The use of smokeless tobacco (ST) ‘Gutkha’ becomes a major public health challenge due to its strong potentiality for genotoxic and cytotoxic effects.

Traditional plant based remedies in health care may be useful in this regard. Arecanut as an emerging etiology of oral cancers in India. AN is known to produce mutagenic and genotoxic effects on tissues of body which may lead to various neoplastic and preneoplastic lesions.

Arecaidine is more potent, cytotoxic, and mutagenic and is tumor promoter. Full-Text Paper (PDF): Cytotoxic and Genotoxic Effects of Cassava Effluents using the Allium cepa Assay. Cytotoxic and Genotoxic Effects of Gutkha Using Allium Cepa Assay Experimental Evolution of Saccharomyces Cerevisiae and Whole-Genome Sequencing: Discovery of Alleles Responsible for Adaptation Finance Essay.

Cytotoxic and genotoxic effects of gutkha
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